@article{111166,
  author = {Lourdes S{\'a}nchez-Cid and M{\`o}nica Pons and Juan Jos{\'e} Lozano and Nuria Rubio and Marta Guerra-Rebollo and Aroa Soriano and Laia Paris-Coderch and Miquel Segura and Raquel Fueyo and Judit Arguimbau and Erika Zodda and Raquel Bermudo and Immaculada Alonso and Xavier Caparr{\'o}s and Marta Cascante and Arash Rafii and Yibin Kang and Marian Mart{\'\i}nez-Balb{\'a}s and Stephen Weiss and Jer{\'o}nimo Blanco and Montserrat Mu{\~n}oz and Pedro Fern{\'a}ndez and Timothy Thomson},
  title = {MicroRNA-200, associated with metastatic breast cancer, promotes traits of mammary luminal progenitor cells},
  abstract = {<p>MicroRNAs are critical regulators of gene networks in normal and abnormal biological processes. Focusing on invasive ductal breast cancer (IDC), we have found dysregulated expression in tumor samples of several microRNAs, including the miR-200 family, along progression from primary tumors to distant metastases, further reflected in higher blood levels of miR-200b and miR-7 in IDC patients with regional or distant metastases relative to patients with primary node-negative tumors. Forced expression of miR-200s in MCF10CA1h mammary cells induced an enhanced epithelial program, aldehyde dehydrogenase (ALDH) activity, mammosphere growth and ability to form branched tubuloalveolar structures while promoting orthotopic tumor growth and lung colonization. MiR-200s also induced the constitutive activation of the PI3K-Akt signaling through downregulation of PTEN, and the enhanced mammosphere growth and ALDH activity induced in MCF10CA1h cells by miR-200s required the activation of this signaling pathway. Interestingly, the morphology of tumors formedby cells expressing miR-200s was reminiscent of metaplastic breast cancer (MBC). Indeed, the epithelial components of MBC samples expressed significantly higher levels of miR-200s than their mesenchymal components and displayed a marker profile compatible with luminal progenitor cells. We propose that microRNAs of the miR-200 family promote traits of highly proliferative breast luminal progenitor cells, thereby exacerbating the growth and metastatic properties of transformed mammary epithelial cells.</p>
},
  year = {2017},
  journal = {Oncotarget},
  volume = {8},
  pages = {83384-83406},
  month = {10/2017},
  issn = {1949-2553},
  doi = {10.18632/oncotarget.20698},
  language = {eng},
}